WNT-mediated signaling relay in stem cells and oncogenesis - from basic biology to applications

Publications

1.      Hsp90-Tau complex reveals molecular basis for specificity in chaperone action. Karagöz GEDuarte AMAkoury EIppel HBiernat JMorán Luengo TRadli MDidenko TNordhues BAVeprintsev DBDickey CA, Mandelkow EZweckstetter MBoelens RMadl T and Rüdiger SG. Cell. 2014 Feb 27;156(5):963-74. 10.1016/j.cell.2014.01.037

2.      Wnt signalling induces accumulation of phosphorylated β-catenin in two distinct cytosolic complexes. Gerlach JP, Emmink BL, Nojima H, Kranenburg O, Maurice MM. Open Biol, 2014 Nov;4(11):140120. 10.1098/rsob.140120.

3.      The plastic liver: differentiated cells, stem cells, every cell? Hindley CJ, Mastrogiovanni G and Huch M, J Clin Invest, 2014 Dec;124(12):5099-102. 10.1172/JCI78372

4.      Asymmetry of VANGL2 in migrating lymphocytes as a tool to monitor activity of the mammalian WNT/planar cell polarity pathway. Kaucká M, Petersen J, Janovská P, Radaszkiewicz T, Smyčková L, Daulat AM, Borg JP, Schulte G, Bryja V., Cell Commun Signal, 2015 Jan 28;13:2. 10.1186/s12964-014-0079-1

5.      Hsp90 interaction with clients. Karagöz GE and Rüdiger SG. Trends Biochem Sci. 2015 Feb;40(2):117-25. 10.1016/j.tibs.2014.12.002

6.      Simplified protocol for flow cytometry analysis of fluorescently labeled exosomes and microvesicles using dedicated flow cytometer. Pospichalova V, Svoboda J, Dave Z, Kotrbova A, Kaiser K, Klemova D, Ilkovics L, Hampl A, Crha I, Jandakova E, Minar L, Weinberger V, Bryja V. J Extracell Vesicles, 2015 Mar 31;4:25530. 10.3402/jev.v4.25530.

7.      Frizzleds and WNT/β-catenin signaling - The black box of ligand-receptor selectivity, complex stoichiometry and activation kinetics. Schulte G. Eur J Pharmacol, 2015 May 21. pii: S0014-2999(15)30015-7. 10.1016/j.ejphar.2015.05.031

8.      Loss-of-Function Mutations in the WNT Co-receptor LRP6 Cause Autosomal-Dominant Oligodontia. Massink MPCréton MASpanevello FFennis WMCune MSSavelberg SMNijman IJMaurice MMvan den Boogaard MJvan Haaften GAm J Hum Genet. 2015 Oct 1;97(4):621-6. 10.1016/j.ajhg.2015.08.014

9.      A script to highlight hydrophobicity and charge on protein surfaces. Hagemans D, van Belzen IA, Morán Luengo T, Rüdiger SG. Front Mol Biosci. 2015 Oct 13;2:56. 10.3389/fmolb.2015.00056

10.  Visualization of a short-range Wnt gradient in the intestinal stem-cell niche. Henner F. Farin, Ingrid Jordens, Mohammed H. Mosa, Onur Basak, Jeroen Korving, Daniele V. F. Tauriello, Karin de Punder, Stephane Angers, Peter J. Peters, Madelon M. MauriceHans Clevers. Nature 2016 Feb 18; 530:340-343. 10.1038/nature16937

11.  Axin cancer mutants form nanoaggregates to rewire the Wnt signaling network. Anvarian ZNojima Hvan Kappel ECMadl TSpit MViertler MJordens ILow TYvan Scherpenzeel RCKuper IRichter KHeck AJ, Boelens RVincent JPRüdiger SGMaurice MM. Nat Struct Mol  Biol. 2016 Apr;23(4):324-32. 10.1038/nsmb.3191.

12.  Dishevelled is a NEK kinase substrate controlling dynamics of centrosomal linker proteins. Cervenka IValnohova JBernatik OHarnos JRadsetoulal MSedova KHanakova KPotesil DSedlackova MSalasova ASteinhart Z,Angers SSchulte GHampl AZdrahal ZBryja VProc Natl Acad Sci U S A. 2016 Aug 16;113(33):9304-9. 10.1073/pnas.1608783113

13.  WNT Stimulation Dissociates a Frizzled 4 Inactive-State Complex with Gα12/13. Arthofer E, Hot B, Petersen J, Strakova K, Jäger S, Grundmann M, Kostenis E, Gutkind JS, Schulte G. Mol Pharmacol. 2016 Oct;90(4):447-59. 10.1124/mol.116.10491

14.  Simultaneous paralogue knockout using a CRISPR-concatemer in mouse small intestinal organoids. Andersson-Rolf A, Merenda A, Mustata RC, Li T, Dietmann S, Koo BK. Simultaneous paralogue knockout using a CRISPR-concatemer in mouse small intestinal organoids. Dev Biol. 2016 Oct 27;240: 271-77. 10.1016/j.ydbio.2016.10.016.

15.  One-step generation of conditional and reversible gene knockouts. Andersson-Rolf A*, Mustata RC*, Merenda A*, Kim J, Perera S, Grego T, Andrews K, Fink J, Skarnes WC and Koo BK. Nat Methods. 2017 Mar;14(3):287-289. *Authors contributed equally to the work. 10.1038/nmeth.4156

16.  FRET Studies of Quinolone-Based Bitopic Ligands and Their Structural Analogues at the Muscarinic M1 Receptor. Messerer R, Kauk M, Volpato D, Alonso Canizal MC, Klöckner J, Zabel U, Nuber S, Hoffmann C, Holzgrabe U. ACS Chemical Biology. 2017 Mar 17;12(3):833-843 10.1021/acschembio.6b00828

17.  FZD10-Gα13 signalling axis points to a role of FZD10 in CNS angiogenesis. Hot B, Valnohova J, Arthofer E, Simon K, Shin J, Uhlén M, Kostenis E, Mulder J, Schulte G. Cell Signal. 2017 Apr;32:93-103. 10.1016/j.cellsig.2017.01.023

18.  A Novel Role for the BMP Antagonist Noggin in Sensitizing Cells to Non-canonical Wnt-5a/Ror2/Disheveled Pathway Activation. Bernatik O, Radaszkiewicz T, Behal M, Dave Z, Witte F, Mahl A, Cernohorsky NH, Krejci P, Stricker S, Bryja V. Front Cell Dev Biol. 2017 May 4;5:47. 10.3389/fcell.2017.00047

19.  A Photoswitchable Dualsteric Ligand Controlling Receptor Efficacy. Agnetta L, Kauk M, Canizal MCA, Messerer R, Holzgrabe U, Hoffmann C, Decker M. Angewandte Chemie International Edition. 2017 Jun 12;56(25):7282-7287. 10.1002/anie.201701524

20.  Molecular regulation and pharmacological targeting of the β-catenin destruction complex. van Kappel EC, Maurice MM. Br J Pharmacol. 2017 Jun 20. 10.1111/bph.13922

21.  The tyrosine Y2502.39 in Frizzled 4 defines a conserved motif important for structural integrity of the receptor and recruitment of Disheveled. Strakova K, Matricon P, Yokota C, Arthofer E, Bernatik O, Rodriguez D, Arenas E, Carlsson J, Bryja V, Schulte G. Cell Signal. 2017 Jun 29;38:85-96. 10.1016/j.cellsig.2017.06.018

22.  N-terminal part of Dishevelled DEP domain is required for Wnt/β-catenin signaling in mammalian cells. Paclíková P, Bernatík O, Radaszkiewicz TW, Bryja V. Mol Cell Biol. 2017 Jul 3. 10.1128/MCB.00145-17

23.  A protocol for multiple gene knockout in mouse small intestinal organoids using a CRISPR-concatemer. Merenda A, Andersson-Rolf A, Mustata RC, Li T, Kim H, Koo BK. J Vis Exp. 2017 Jul 12;(125). 10.3791/55916

24.  Agonist‐induced dimer dissociation as a macromolecular step in G protein‐coupled receptor signaling. Petersen J, Wright SC, Rodríguez D, Matricon P, Lahav N, Vromen A, Friedler A, Strömqvist J, Wennmalm S, Carlsson J, Schulte G. Nat Commun. 2017 Aug 9;8(1):226. 10.1038/s41467-017-00253-9.